FastQCFastQC Report
Sat 18 Mar 2023
SRR4895540_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895540_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1412189
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA73330.5192647726331249No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG69880.4948346149134429No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC51400.36397394399758104No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCCGGGCGCGGTGGCTCAC33720.23877823719063102No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCCAGGCATGGTGGCTCA27600.19544126175745596No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCGGGCGCGGTGGCTCACG24400.1727814053218089No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCCGGGCGCAGTGGCTCAC22660.1604601083849258No Hit
GAGGAGGCTACTGGCGTCGAGCCCACTGCCTCAGAAGACTTCCTTTCATC21000.1487053078589339No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCGGGGAGGTAGTGACGAAAA19480.13794187605200153No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAATTCTGGACATTAATTA17670.12512489475558866No Hit
GTCGAGCCCACTGCCTCAGAAGACTTCCTTTCATCTGTTCCTACTGAGCT17350.12285890911202396No Hit
CCACTGCCTCAGAAGACTTCCTTTCATCTGTTCCTACTGAGCTGGTCCCA17090.12101779577662762No Hit
GGTGGTCACTGCGTGGCCCTGTGTACCCGAGGAGGCTACTGGCGTCGAGC16940.11995561500620668No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAATTCTGGACATTAATT16240.11499877141090888No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCGGGCGCAGTGGCTCACG15930.11280359781870558No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCCGGGCGCGGTGGCTCA15730.11138735679147763No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT284150.0138.617871
GTGGTAT285000.0137.83126
GGTATCA285850.0137.396188
TGGTATC287150.0136.7247
GTATCAA288500.0136.583349
AGCAGTG289400.0135.732
AGTGGTA291400.0134.952275
CAGTGGT291450.0134.879714
GCAGTGG298100.0131.720413
CAACGCA286900.028.12147710-14
ATCAACG285750.028.09859510-14
AGAGTAC286850.028.03100415-19
TCAACGC286900.028.01607710-14
TACATGG284450.028.00427420-24
CAGAGTA288750.027.84655815-19
GTACATG287350.027.78679320-24
CGCAGAG289050.027.7678415-19
GCAGAGT290300.027.61355215-19
AGTACAT291150.027.56260920-24
AACGCAG293850.027.47596410-14
Produced by FastQC (version 0.11.5)