FastQCFastQC Report
Sat 18 Mar 2023
SRR4895539_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895539_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3039648
Sequences flagged as poor quality0
Sequence length150
%GC33

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAACGA3041250.0143.91622
GCTAACG3041300.0143.913831
TAACGAG1084350.0143.728733
TAACGAT1077250.0143.72033
TAACGAA879900.0143.54433
AACGATG386500.0143.305084
AACGAGT372600.0143.144124
AACGATT366100.0142.873374
AACGATA327550.0142.829354
AACGAAT308850.0142.828754
ACGATAG117550.0142.58565
ACGATTG135350.0142.55815
AACGAGG405400.0142.431494
AACGAGA309300.0142.36494
AACGAAG326700.0142.341494
ACGATTA107550.0141.91915
ACGATAT113850.0141.780985
ACGATGA106950.0141.638185
ACGAATG108350.0141.602175
ACGAAGT113350.0141.580645
Produced by FastQC (version 0.11.5)