FastQCFastQC Report
Sat 18 Mar 2023
SRR4895539_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895539_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3039648
Sequences flagged as poor quality0
Sequence length150
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA157340.5176257250839571No Hit
GAGGAGGCTACTGGCGTCGAGCCCACTGCCTCAGAAGACTTCCTTTCATC72020.23693532935392517No Hit
GTCGAGCCCACTGCCTCAGAAGACTTCCTTTCATCTGTTCCTACTGAGCT57610.1895285243554517No Hit
CCACTGCCTCAGAAGACTTCCTTTCATCTGTTCCTACTGAGCTGGTCCCA56960.18739011885586754No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC56600.18620577119455936No Hit
GGTGGTCACTGCGTGGCCCTGTGTACCCGAGGAGGCTACTGGCGTCGAGC55990.184198959879565No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG54340.178770699765236No Hit
CTCAGAAGACTTCCTTTCATCTGTTCCTACTGAGCTGGTCCCAGCCAGCA48710.16024881828422238No Hit
TGGAACTTTGCGTACTGGGCTGCTCACCAGCCCTGGTCCCGCGGTGGTCA44740.14718809546368528No Hit
AGAAGACTTCCTTTCATCTGTTCCTACTGAGCTGGTCCCAGCCAGCAGTT44270.1456418637947552No Hit
GAGCCCACTGCCTCAGAAGACTTCCTTTCATCTGTTCCTACTGAGCTGGT43780.1440298350335302No Hit
AAGACTTCCTTTCATCTGTTCCTACTGAGCTGGTCCCAGCCAGCAGTTCA43750.14393113939508786No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT42540.13995041531124655No Hit
GTGGCCCTGTGTACCCGAGGAGGCTACTGGCGTCGAGCCCACTGCCTCAG40930.13465374938150734No Hit
GGCAGCCGCTGGAACTTTGCGTACTGGGCTGCTCACCAGCCCTGGTCCCG40590.1335351988124941No Hit
AGCCGCTGGAACTTTGCGTACTGGGCTGCTCACCAGCCCTGGTCCCGCGG40500.13323911189716706No Hit
CGCTGGAACTTTGCGTACTGGGCTGCTCACCAGCCCTGGTCCCGCGGTGG40130.13202186568971144No Hit
CACTGCCTCAGAAGACTTCCTTTCATCTGTTCCTACTGAGCTGGTCCCAG39380.12955447472865278No Hit
GAAGACTTCCTTTCATCTGTTCCTACTGAGCTGGTCCCAGCCAGCAGTTC37720.12409331606817631No Hit
AGCCCACTGCCTCAGAAGACTTCCTTTCATCTGTTCCTACTGAGCTGGTC37300.12271157712998347No Hit
CAGAAGACTTCCTTTCATCTGTTCCTACTGAGCTGGTCCCAGCCAGCAGT37100.12205360620703448No Hit
AGACTTCCTTTCATCTGTTCCTACTGAGCTGGTCCCAGCCAGCAGTTCAG36710.120770562907284No Hit
CGAGCCCACTGCCTCAGAAGACTTCCTTTCATCTGTTCCTACTGAGCTGG36280.11935592542294371No Hit
CGAGGAGGCTACTGGCGTCGAGCCCACTGCCTCAGAAGACTTCCTTTCAT35940.11823737485393046No Hit
AGCCCTGGTCCCGCGGTGGTCACTGCGTGGCCCTGTGTACCCGAGGAGGC35640.117250418469507No Hit
AGGATCACAGGCTCGGGTCGCTGCAGACGCTTTCAGTGGGTTGACGGCAG35420.11652665045426312No Hit
TGCTCACCAGCCCTGGTCCCGCGGTGGTCACTGCGTGGCCCTGTGTACCC35130.11557259261598711No Hit
AGACGCTTTCAGTGGGTTGACGGCAGCCGCTGGAACTTTGCGTACTGGGC35020.11521070860836519No Hit
GTACTGGGCTGCTCACCAGCCCTGGTCCCGCGGTGGTCACTGCGTGGCCC34990.11511201296992284No Hit
GCTGGAACTTTGCGTACTGGGCTGCTCACCAGCCCTGGTCCCGCGGTGGT34660.11402636094705702No Hit
ACCAGCCCTGGTCCCGCGGTGGTCACTGCGTGGCCCTGTGTACCCGAGGA34120.11224983945509481No Hit
CCTGGTCCCGCGGTGGTCACTGCGTGGCCCTGTGTACCCGAGGAGGCTAC34020.11192085399362031No Hit
AGGAGGCTACTGGCGTCGAGCCCACTGCCTCAGAAGACTTCCTTTCATCT32820.10797302845592648No Hit
CCTGTGTACCCGAGGAGGCTACTGGCGTCGAGCCCACTGCCTCAGAAGAC32590.10721636189453515No Hit
ACTGGCGTCGAGCCCACTGCCTCAGAAGACTTCCTTTCATCTGTTCCTAC32550.10708476770994535No Hit
ATCTGTTCCTACTGAGCTGGTCCCAGCCAGCAGTTCAGAGCTGCCCTCTC32070.10550563749486781No Hit
CCCACTGCCTCAGAAGACTTCCTTTCATCTGTTCCTACTGAGCTGGTCCC31220.10270926107233469No Hit
GGCCCTGTGTACCCGAGGAGGCTACTGGCGTCGAGCCCACTGCCTCAGAA30650.10083404394193013No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT400050.0137.718631
GGTATCA398800.0137.49818
GTGGTAT399250.0137.415246
GTATCAA403250.0136.516399
TGGTATC402600.0136.164527
AGTGGTA407350.0134.788835
CAGTGGT410700.0133.654344
AGCAGTG410850.0133.555162
GCAGTGG425800.0128.815263
ATCAACG398900.028.18220510-14
TCAACGC399950.028.16222610-14
AGAGTAC400400.028.06602515-19
TACATGG398200.027.9361420-24
CAGAGTA402300.027.92631315-19
CAACGCA407150.027.71725810-14
GTACATG404700.027.56572720-24
AGTACAT407950.027.42730920-24
GAGTACA400950.027.3566520-24
GCAGAGT410550.027.33707615-19
CGCAGAG411600.027.3058215-19
Produced by FastQC (version 0.11.5)