FastQCFastQC Report
Sat 18 Mar 2023
SRR4895538_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895538_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1919930
Sequences flagged as poor quality0
Sequence length150
%GC32

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCGAC1921200.0143.894152
ATAGCGA1921200.0143.894151
AGCGACT705400.0143.613943
AGCGACG631250.0143.318683
GCGACTA210800.0143.24024
AGCGACA588700.0143.17223
GCGACTT258350.0143.12774
GCGACAT216350.0143.026544
GCGACGT234150.0142.607944
CGACGAT62150.0142.601495
CGACTTA77550.0142.506165
GCGACTG237450.0142.445244
CGACGTA74150.0142.146775
GCGACGA178750.0142.138824
GCGACAA178550.0142.136754
CGACTAA63050.0142.050385
CGACTTG89200.0141.973775
CGACATG70600.0141.850075
GCGACAG195650.0141.71014
CGACTAG70800.0141.652745
Produced by FastQC (version 0.11.5)