FastQCFastQC Report
Sat 18 Mar 2023
SRR4895538_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895538_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1919930
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA97330.5069455657237504No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG53480.2785518222018511No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC41780.21761210044116192No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCCGGGCGCAGTGGCTCAT40330.21005974176141837No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA29440.15333892381493075No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCCGGGCGCGGTGGCTCAC25240.13146312625981155No Hit
GAGGAGGCTACTGGCGTCGAGCCCACTGCCTCAGAAGACTTCCTTTCATC23690.12338991525732709No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAATTCTGGACATTAATTA23550.12266072200548979No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCGGGCGCAGTGGCTCATG23210.12088982410817062No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAATTCTGGACATTAATT20900.10885813545285505No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCCAGGCGCAGTGGCTCAC20250.10547259535503897No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCTTTCCGGCGGTGACGAC19340.10073283921809648No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA249650.0138.048038
AAGCAGT252550.0137.04371
GTGGTAT251850.0136.674186
GTATCAA253800.0136.414839
TGGTATC253300.0135.977087
CAGTGGT257100.0134.023324
AGCAGTG258900.0133.015012
AGTGGTA259650.0132.651635
GCAGTGG275550.0124.974383
AGAGTAC250550.028.14907315-19
ATCAACG251200.028.14428710-14
TCAACGC252600.028.05100410-14
CAACGCA254600.027.92679610-14
CAGAGTA252650.027.90939915-19
GTACATG250450.027.83535220-24
TACATGG250150.027.81692520-24
AGTACAT252950.027.69118720-24
CGCAGAG258150.027.3649815-19
GAGTACA250950.027.34950820-24
GCAGAGT257600.027.30043215-19
Produced by FastQC (version 0.11.5)