FastQCFastQC Report
Sat 18 Mar 2023
SRR4895537_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895537_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3018664
Sequences flagged as poor quality0
Sequence length150
%GC32

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGCTAA3020500.0143.902661
GGCTAAC3020800.0143.888382
GCTAACG1039600.0143.796943
GCTAACT1085350.0143.791853
GCTAACA894650.0143.427553
CTAACGG361700.0143.373814
CTAACTG378100.0143.362494
CTAACGT374450.0143.356374
CTAACGA302950.0143.301684
CTAACTA328300.0142.214554
CTAACAG308550.0141.867514
TAACGGT133400.0141.61625
CTAACTT387750.0141.16864
TAACGTA118800.0141.081955
TAACGAT110850.0141.068185
TAACTAG114850.0140.919225
TAACGGG123550.0140.844155
TAACGGA109200.0140.826225
TAACGAG101550.0140.80055
CTAACAT330100.0140.784764
Produced by FastQC (version 0.11.5)