FastQCFastQC Report
Sat 18 Mar 2023
SRR4895537_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895537_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3018664
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA238320.7894883299366872No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG58800.19478815794006885No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCCGGGCGCGGTGGCTCA53470.177131340222032No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC48200.15967328593046462No Hit
GAGGAGGCTACTGGCGTCGAGCCCACTGCCTCAGAAGACTTCCTTTCATC38520.12760611979339204No Hit
GTCGAGCCCACTGCCTCAGAAGACTTCCTTTCATCTGTTCCTACTGAGCT32080.10627217868567021No Hit
GGTGGTCACTGCGTGGCCCTGTGTACCCGAGGAGGCTACTGGCGTCGAGC31210.10339010900186309No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30240.10017676694060683No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA357550.0137.344338
GTGGTAT359350.0136.596276
TGGTATC361950.0135.615057
GTATCAA363700.0135.45749
AAGCAGT363350.0135.451421
AGTGGTA370100.0132.725925
AGCAGTG371800.0131.98572
CAGTGGT373700.0131.331734
GCAGTGG384900.0127.456183
ATCAACG357700.028.04945610-14
TCAACGC358650.028.03136410-14
AGAGTAC356700.028.0159915-19
CAGAGTA357000.027.97227915-19
TACATGG358300.027.72656820-24
GTACATG361450.027.51282320-24
GAGTACA356050.027.42455320-24
AGTACAT364900.027.3237320-24
CAACGCA371850.027.14085210-14
GCAGAGT367650.027.11890215-19
TATCAAC367950.026.81413310-14
Produced by FastQC (version 0.11.5)