FastQCFastQC Report
Sat 18 Mar 2023
SRR4895536_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895536_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences993939
Sequences flagged as poor quality0
Sequence length150
%GC34

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCATGC994900.0143.847822
AGCCATG995150.0143.811691
CCATGCG363300.0143.457473
CCATGCT337650.0143.374193
CCATGCA295400.0143.26143
CATGCGA103600.0143.228124
CATGCTA99100.0143.19344
CATGCGT125900.0143.020424
CATGCGG133850.0142.755424
CATGCTG122500.0142.523244
CATGCAG104100.0142.471014
ATGCGTA39850.0142.366555
ATGCGAT35300.0142.360925
ATGCGGA39200.0142.155935
CATGCAA89500.0142.142364
CATGCAT103500.0141.836144
ATGCTGA36400.0141.816855
ATGCGAA31600.0141.714195
ATGCGAG37550.0141.691765
ATGCTAA27800.0141.661745
Produced by FastQC (version 0.11.5)