FastQCFastQC Report
Sat 18 Mar 2023
SRR4895536_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895536_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences993939
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA46930.4721617725031415No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCCGGGCGCAGTGGCTCAT31710.3190336630316348No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC27360.275268401783208No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAATTCTGGACATTAATT24350.2449848531952162No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG24300.2444818042153492No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAATTCTGGACATTAATTA18450.1856250735709133No Hit
GAGGAGGCTACTGGCGTCGAGCCCACTGCCTCAGAAGACTTCCTTTCATC15920.16017079518964444No Hit
GGTGGTCACTGCGTGGCCCTGTGTACCCGAGGAGGCTACTGGCGTCGAGC12730.12807627027413152No Hit
CCACTGCCTCAGAAGACTTCCTTTCATCTGTTCCTACTGAGCTGGTCCCA12510.12586285476271683No Hit
GTCGAGCCCACTGCCTCAGAAGACTTCCTTTCATCTGTTCCTACTGAGCT12080.12153663353586086No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCGGGCGCAGTGGCTCATG11480.11550004577745718No Hit
CTCAGAAGACTTCCTTTCATCTGTTCCTACTGAGCTGGTCCCAGCCAGCA10150.10211894291299567No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA131950.0136.957588
GTGGTAT131950.0136.684756
GTATCAA133800.0135.97879
AAGCAGT134750.0134.913161
TGGTATC134100.0134.815447
AGCAGTG135400.0133.467882
CAGTGGT136650.0132.141624
AGTGGTA136900.0132.00555
GCAGTGG143100.0126.1855553
CCGTTAG200.006285220428.80044435-39
ATCAACG131850.028.10032710-14
AGAGTAC131900.028.07875815-19
TCAACGC133350.027.8490310-14
CAACGCA133800.027.84146110-14
GTACATG131850.027.80545220-24
CAGAGTA133100.027.7931515-19
TACATGG131700.027.7387220-24
AGTACAT133950.027.52003520-24
GAGTACA132450.027.32072820-24
CGCAGAG135500.027.29024515-19
Produced by FastQC (version 0.11.5)