FastQCFastQC Report
Sat 18 Mar 2023
SRR4895535_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895535_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1428328
Sequences flagged as poor quality0
Sequence length150
%GC34

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACCT1430650.0143.758291
GCACCTC1433900.0143.432462
ACCTCGA139100.0143.218434
CACCTCG497400.0142.836853
ACCTCGT176650.0142.160744
ACCTCGG181350.0142.089174
ACCTCTA153700.0142.027424
CACCTCA440150.0141.99923
ACCTCAG145050.0141.810844
CACCTCT508050.0141.217243
ACCTCAT156300.0141.000694
ACCTCTG173100.0140.916934
CCTCGAT53050.0140.601915
ACCTCAA140800.0139.648394
CCTCGTA53900.0139.586825
CCTCGAG45250.0139.06245
CCTCTAG54800.0138.870915
CCTCGGA52700.0138.803365
CTCGGAT17700.0137.893346
CTCGAAT16000.0137.695046
Produced by FastQC (version 0.11.5)