FastQCFastQC Report
Sat 18 Mar 2023
SRR4895535_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895535_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1428328
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA97860.6851367473017402No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG50750.3553105449168538No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC43790.306582241613971No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAATTCTGGACATTAATT21710.15199590010137728No Hit
GAGGAGGCTACTGGCGTCGAGCCCACTGCCTCAGAAGACTTCCTTTCATC18380.12868192740042905No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCCAGGCGCGGTGGCTCA17320.121260662816944No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAATTCTGGACATTAATTA16780.11748001859516863No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCCGGGCGTGGTGGCTCA16540.11579973227437955No Hit
GTCGAGCCCACTGCCTCAGAAGACTTCCTTTCATCTGTTCCTACTGAGCT14840.10389770416879035No Hit
CCACTGCCTCAGAAGACTTCCTTTCATCTGTTCCTACTGAGCTGGTCCCA14680.10277751328826432No Hit
GGTGGTCACTGCGTGGCCCTGTGTACCCGAGGAGGCTACTGGCGTCGAGC14530.10172733433777116No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA189150.0137.944878
AAGCAGT191500.0136.552861
GTGGTAT191300.0136.206346
GTATCAA192600.0136.034649
TGGTATC193600.0134.848517
AGCAGTG196150.0132.875232
AGTGGTA196900.0132.36915
CAGTGGT197200.0132.240744
GCAGTGG201350.0129.407873
CGTCAAT950.00489121230.315183
ATCAACG189300.028.10719910-14
AGAGTAC189200.027.96242115-19
CAGAGTA189650.027.90366615-19
TCAACGC191550.027.85221910-14
TACATGG189100.027.80284320-24
CAACGCA192550.027.75991810-14
GTACATG189650.027.69184320-24
AGTACAT191900.027.47221620-24
GAGTACA189450.027.37142820-24
GCAGAGT193200.027.30150615-19
Produced by FastQC (version 0.11.5)