FastQCFastQC Report
Sat 18 Mar 2023
SRR4895534_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895534_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences708116
Sequences flagged as poor quality0
Sequence length150
%GC32

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA232513.2835015731885733No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATAC61060.8622880996898813No Hit
AGATGTACTCTGCGTTGATACCACTGCTTAGATCGGAAGAGCGTCGTGTA7390.10436143230770101Illumina Single End PCR Primer 1 (100% over 21bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATGTAC709650.0143.677442
AGATGTA710400.0143.525761
ATGTACG249800.0142.637343
TGTACGA66650.0142.479664
TGTACGT89550.0142.3844
ATGTACA190400.0142.176933
GTACGTA26600.0142.097325
GTACGGT34800.0141.92315
TGTACGG94100.0141.31414
GTACGAA21250.0140.942725
TGTACAG67000.0140.660784
TGTACTG85750.0140.633544
TGTACTA67550.0140.581334
GTACTGA25300.0140.577125
GTACGTT32550.0140.452975
GTACGAT24100.0140.407095
TACGTAG10000.0140.392156
GTACAGT23950.0140.384645
GTACGGG35450.0140.33635
GTACTGG33300.0140.316475
Produced by FastQC (version 0.11.5)