FastQCFastQC Report
Sat 18 Mar 2023
SRR4895534_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895534_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences708116
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG205752.9055973879985766No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA53100.7498771387738732No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA37080.5236430189403997No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC19100.2697298182783612No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG17400.24572245225358558No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAATTCTGGACATTAATT9890.1396663823441357No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAATTCTGGACATTAATTA9000.12709782013116494No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT199250.087.586841
AGTGGTA210700.082.724635
GTATCAA211450.082.70369
GGTATCA211400.082.382598
AGCAGTG213450.081.5239262
CAGTGGT214650.081.135254
TGGTATC214750.081.063947
GTGGTAT215050.080.917376
GCAGTGG218150.079.734513
CGCAGAT5050.024.52292890-94
CGACCTA1200.023.99981345-49
AACGCGA1600.022.49982845-49
AAACCGC5600.022.11442885-89
ATGGGGG24600.022.00989725-29
GAAACCG5850.021.41552485-89
CGATAGG555.5373403E-620.94617835-39
CATGGGG63450.020.87956425-29
CGCGAAA709.4145435E-820.5715690-94
CGGTAAC651.0519234E-619.93858785-89
TACGCAC1450.019.86275750-54
Produced by FastQC (version 0.11.5)