FastQCFastQC Report
Sat 18 Mar 2023
SRR4895533_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895533_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3774381
Sequences flagged as poor quality0
Sequence length150
%GC31

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATTCG1289250.0143.797843
CAGATTC3779650.0143.791372
ACAGATT3782850.0143.669721
AGATTCA1133350.0143.459693
GATTCGA378100.0143.422034
GATTCAG381400.0143.068314
AGATTCT1360250.0143.056433
GATTCGT483150.0143.039584
GATTCGG430650.0142.873174
GATTCAT406800.0142.719014
GATTCAA348250.0142.422074
GATTCTA413350.0142.390844
GATTCTG440900.0142.376684
ATTCGTA148000.0141.998785
ATTCGAT142950.0141.374315
ATTCGAA117300.0141.354025
ATTCGGA122600.0141.174485
ATTCGAG123250.0141.072535
ATTCTAG144150.0139.847785
ATTCGGT163300.0139.760795
Produced by FastQC (version 0.11.5)