FastQCFastQC Report
Sat 18 Mar 2023
SRR4895532_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895532_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3470962
Sequences flagged as poor quality0
Sequence length150
%GC33

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGAC3478800.0143.670261
ACGACCT1205200.0143.636753
CACGACC3481250.0143.569152
ACGACCG1214550.0143.53283
CGACCTA366750.0143.308094
CGACCAT368400.0143.27214
ACGACCA1055000.0143.210373
CGACCGT432750.0143.01364
CGACCTT427500.0142.816284
CGACCGA355800.0142.760834
GACCGAT124450.0142.201775
CGACCTG415700.0142.124664
GACCTAT130800.0142.123695
CGACCAG365100.0142.082354
GACCGTA133500.0142.053685
CGACCAA324300.0141.97494
GACCATG128600.0141.64385
GACCGTT160300.0141.569815
GACCTAG123100.0141.538735
GACCTAA116650.0141.40295
Produced by FastQC (version 0.11.5)