FastQCFastQC Report
Sat 18 Mar 2023
SRR4895531_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895531_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1079637
Sequences flagged as poor quality0
Sequence length150
%GC33

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGTTGC1081800.0143.703462
AATGTTG1084000.0143.41181
TGTTGCA326500.0142.386553
GTTGCGA105550.0142.290994
GTTGCTA114500.0141.984124
GTTGCAT119100.0141.820024
TGTTGCG382350.0141.5863
GTTGCAG112550.0140.861774
TGTTGCT390550.0139.79313
GTTGCAA97500.0139.787174
TGCGATA12550.0139.406776
TTGCGAT40100.0138.9695
GTTGCGT144200.0138.90354
GTTGCGG137000.0138.005224
TTGCGTA44550.0137.693425
TTGCGGA38200.0137.588095
TTGCTAG43600.0137.225815
TGCGAAT12650.0136.597276
GTTGCTG135850.0136.523534
TGCTAGG15750.0136.225076
Produced by FastQC (version 0.11.5)