FastQCFastQC Report
Sat 18 Mar 2023
SRR4895531_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895531_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1079637
Sequences flagged as poor quality0
Sequence length150
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA54520.5049845457315746No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG23500.21766575247050632No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC19840.18376546931978063No Hit
GAGGAGGCTACTGGCGTCGAGCCCACTGCCTCAGAAGACTTCCTTTCATC17570.1627398838683743No Hit
GGTGGTCACTGCGTGGCCCTGTGTACCCGAGGAGGCTACTGGCGTCGAGC14310.13254454969587No Hit
CCACTGCCTCAGAAGACTTCCTTTCATCTGTTCCTACTGAGCTGGTCCCA14310.13254454969587No Hit
GTCGAGCCCACTGCCTCAGAAGACTTCCTTTCATCTGTTCCTACTGAGCT14280.1322666785225034No Hit
TGGAACTTTGCGTACTGGGCTGCTCACCAGCCCTGGTCCCGCGGTGGTCA11940.1105927269999083No Hit
CTCAGAAGACTTCCTTTCATCTGTTCCTACTGAGCTGGTCCCAGCCAGCA11750.10883287623525316No Hit
AGCCGCTGGAACTTTGCGTACTGGGCTGCTCACCAGCCCTGGTCCCGCGG11060.10244183924782126No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA135600.0135.341788
GTGGTAT135850.0134.986726
AAGCAGT137300.0134.051731
TGGTATC137000.0133.85367
GTATCAA138050.0133.513539
AGTGGTA139200.0131.686395
CAGTGGT141550.0129.703614
AGCAGTG142750.0128.479812
GCAGTGG144350.0126.9559563
AGAGTAC136850.027.62079815-19
ATCAACG136500.027.5755810-14
TCAACGC137350.027.488810-14
CAGAGTA137050.027.48593115-19
TACATGG136700.027.2929620-24
GTACATG137000.027.25421320-24
CAACGCA139550.027.1895810-14
AGTACAT138250.027.10153420-24
CGCAGAG140750.026.74292615-19
GAGTACA137000.026.70765920-24
AACGCAG142800.026.65144210-14
Produced by FastQC (version 0.11.5)