FastQCFastQC Report
Sat 18 Mar 2023
SRR4895530_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895530_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1424836
Sequences flagged as poor quality0
Sequence length150
%GC35

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCCGTC1426150.0143.857272
AATCCGT1426450.0143.827011
TCCGTCA438700.0143.255073
TCCGTCG486200.0143.10513
CCGTCTA152450.0142.765694
CCGTCAA135600.0142.66624
CGTCTAA43600.0142.50745
TCCGTCT508800.0142.394593
CGTCGAT53250.0142.371115
CGTCAAT49700.0142.255225
CCGTCAG145150.0142.108724
CGTCAAG45100.0141.918295
CCGTCGA141100.0141.901554
CGTCGAA42900.0141.811865
CGTCATA47900.0141.738985
CCGTCAT159700.0141.694374
CGTCTTA54950.0141.504155
CGTCAGA41700.0141.403765
CGTCGTA58600.0141.290635
CGTCTAG52300.0141.102695
Produced by FastQC (version 0.11.5)