FastQCFastQC Report
Sat 18 Mar 2023
SRR4895530_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895530_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1424836
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA74380.5220249909463265No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG51330.36025198689533394No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC42150.2958235193383659No Hit
GAGGAGGCTACTGGCGTCGAGCCCACTGCCTCAGAAGACTTCCTTTCATC23200.16282575678885147No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAATTCTGGACATTAATTA20930.14689409868925266No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAATTCTGGACATTAATT20340.1427532712536741No Hit
GGTGGTCACTGCGTGGCCCTGTGTACCCGAGGAGGCTACTGGCGTCGAGC18410.12920785269322224No Hit
GTCGAGCCCACTGCCTCAGAAGACTTCCTTTCATCTGTTCCTACTGAGCT18180.1275936318285052No Hit
CCACTGCCTCAGAAGACTTCCTTTCATCTGTTCCTACTGAGCTGGTCCCA18180.1275936318285052No Hit
CTCAGAAGACTTCCTTTCATCTGTTCCTACTGAGCTGGTCCCAGCCAGCA14920.10471380565903726No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT240750.0138.861111
GGTATCA241750.0137.949428
GTGGTAT242500.0137.705736
TGGTATC244150.0136.598167
GTATCAA245650.0136.08179
AGTGGTA247700.0134.931125
AGCAGTG247600.0134.699552
CAGTGGT248200.0134.601294
GCAGTGG252950.0131.907533
ATCAACG241300.028.23847810-14
AGAGTAC242000.028.19924215-19
CAACGCA243550.028.08993710-14
TCAACGC243350.028.04793510-14
CAGAGTA243950.027.96202715-19
TACATGG243100.027.78711920-24
GTACATG244150.027.7737820-24
CGCAGAG246200.027.70063615-19
AGTACAT245700.027.68061820-24
GCAGAGT248050.027.45920615-19
GAGTACA242750.027.44753320-24
Produced by FastQC (version 0.11.5)