FastQCFastQC Report
Sat 18 Mar 2023
SRR4895529_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895529_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences916872
Sequences flagged as poor quality0
Sequence length150
%GC31

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGACAC918650.0143.711752
AAGGACA919250.0143.617951
GGACACT334150.0143.519883
GGACACA282550.0143.254943
GACACGT110750.0142.953754
GGACACG303400.0142.926043
GACACTA103650.0142.743594
GACACAT100900.0142.56684
GACACTG105600.0142.425784
GACACTT126700.0142.175494
GACACGG99450.0142.039234
GACACGA93500.0141.452824
GACACAG94000.0141.389744
ACACTGG36200.0140.016165
ACACGGT37600.0139.97285
ACACGAT33950.0139.752555
GACACAA91100.0139.410114
ACACGTA36500.0139.06265
ACACTAG33700.0138.439225
CACGAAT12950.0138.434286
Produced by FastQC (version 0.11.5)