FastQCFastQC Report
Sat 18 Mar 2023
SRR4895529_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895529_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences916872
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA45400.4951618110270572No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAATTCTGGACATTAATTA18340.20002792101841915No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAATTCTGGACATTAATT17160.18715807659084366No Hit
GAGGAGGCTACTGGCGTCGAGCCCACTGCCTCAGAAGACTTCCTTTCATC14560.15880079225889765No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG13390.14604001430952193No Hit
GTCGAGCCCACTGCCTCAGAAGACTTCCTTTCATCTGTTCCTACTGAGCT11820.12891657723215455No Hit
GGTGGTCACTGCGTGGCCCTGTGTACCCGAGGAGGCTACTGGCGTCGAGC10320.1125566055021857No Hit
CCACTGCCTCAGAAGACTTCCTTTCATCTGTTCCTACTGAGCTGGTCCCA10290.11222940606758633No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC9880.10775768046139483No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA102600.0137.750328
GTATCAA103900.0136.858329
AAGCAGT104650.0135.67851
GTGGTAT104800.0134.789926
AGCAGTG105400.0133.824982
TGGTATC105600.0133.700597
AGTGGTA107000.0132.085825
CAGTGGT108350.0130.307174
GCAGTGG108950.0129.464463
TCGTACG800.028.80103350-54
ATCAACG102150.028.24938810-14
AGAGTAC102200.028.20738615-19
TCAACGC103700.027.86880110-14
CAACGCA103950.027.85718710-14
GTACATG103200.027.78057520-24
TACATGG102950.027.75012620-24
CAGAGTA103950.027.74636815-19
AGTACAT104350.027.59860820-24
GAGTACA103000.027.48501220-24
CGCAGAG105500.027.29777115-19
Produced by FastQC (version 0.11.5)