FastQCFastQC Report
Sat 18 Mar 2023
SRR4895528_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895528_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1410204
Sequences flagged as poor quality0
Sequence length150
%GC32

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAA1411150.0143.89352
GCGAGTA1411300.0143.87821
GAGTAAG512350.0143.147313
GAGTAAT504350.0142.862723
GAGTAAA402450.0142.612953
AGTAATG184800.0140.289374
AGTAAGG191500.0139.892524
AGTAAGA147100.0138.949284
AGTAATA151300.0138.565814
AGTAAGT187200.0138.49084
GTAATAG58700.0137.98065
AGTAAAG155150.0137.818744
GTAAGTA57500.0136.85355
GTAAGGA59500.0136.85145
AGTAAAT159150.0136.526264
GTAATGA53900.0136.51045
GTAATGG71400.0136.024575
GTAAGAG53550.0135.520395
GTAAATG59050.0135.090095
GTAAGTG64300.0134.809085
Produced by FastQC (version 0.11.5)