FastQCFastQC Report
Sat 18 Mar 2023
SRR4895528_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895528_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1410204
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA72870.516733749159696No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG27140.19245442503354124No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC23010.16316788209365454No Hit
GAGGAGGCTACTGGCGTCGAGCCCACTGCCTCAGAAGACTTCCTTTCATC22830.16189147102121396No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAATTCTGGACATTAATTA19980.14168162904090473No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAATTCTGGACATTAATT19400.13756874891859616No Hit
GTCGAGCCCACTGCCTCAGAAGACTTCCTTTCATCTGTTCCTACTGAGCT18920.13416498605875463No Hit
CCACTGCCTCAGAAGACTTCCTTTCATCTGTTCCTACTGAGCTGGTCCCA18470.13097395837765316No Hit
GGTGGTCACTGCGTGGCCCTGTGTACCCGAGGAGGCTACTGGCGTCGAGC18440.13076122319891306No Hit
CTCAGAAGACTTCCTTTCATCTGTTCCTACTGAGCTGGTCCCAGCCAGCA15690.11126049848107081No Hit
AGAAGACTTCCTTTCATCTGTTCCTACTGAGCTGGTCCCAGCCAGCAGTT14600.10353112032018062No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCCGGGCGCAGTGGCTCAT14420.10225470924774005No Hit
AAGACTTCCTTTCATCTGTTCCTACTGAGCTGGTCCCAGCCAGCAGTTCA14340.10168741543776647No Hit
GAGCCCACTGCCTCAGAAGACTTCCTTTCATCTGTTCCTACTGAGCTGGT14230.10090738644905277No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA187000.0137.680918
GTGGTAT188900.0136.29616
AAGCAGT190300.0136.206221
GTATCAA190400.0135.751729
TGGTATC190800.0134.938847
AGTGGTA191450.0134.631135
CAGTGGT192450.0134.118624
AGCAGTG193300.0133.45912
GCAGTGG202850.0127.104973
ATCAACG187500.028.13089210-14
TCAACGC188800.028.04396810-14
CAACGCA189200.028.01512110-14
AGAGTAC188750.028.0136515-19
TACATGG186350.027.91238820-24
GTACATG188050.027.79789720-24
CAGAGTA190500.027.69583715-19
AGTACAT190600.027.56954620-24
CGCAGAG192900.027.42590115-19
GAGTACA189000.027.26958720-24
AACGCAG195800.027.1516910-14
Produced by FastQC (version 0.11.5)