FastQCFastQC Report
Sat 18 Mar 2023
SRR4895527_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895527_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1813947
Sequences flagged as poor quality0
Sequence length150
%GC31

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGATC1816150.0143.816242
GAGATCT684700.0143.5533
GAGATCA544450.0143.439223
AAGAGAT1821100.0143.425321
GAGATCG588850.0143.34663
AGATCGA174650.0143.08774
AGATCTG223650.0142.803534
AGATCGT227700.0142.761474
AGATCTA203250.0142.71944
AGATCAT198750.0142.473184
GATCGAT67400.0142.39235
AGATCAG177950.0142.295334
GATCTAG64500.0141.98545
AGATCAA170950.0141.930944
GATCGAG55700.0141.797215
AGATCTT262450.0141.607974
GATCGTA70750.0141.348795
GATCATA61950.0141.32165
GATCTGG75750.0141.23835
GATCAAG53600.0141.173835
Produced by FastQC (version 0.11.5)