FastQCFastQC Report
Sat 18 Mar 2023
SRR4895527_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895527_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1813947
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA98390.5424083504093559No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC37360.20595971106101776No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG33730.18594810101948953No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCCGGGCGCGGTGGCTCA31910.17591473179756628No Hit
GAGGAGGCTACTGGCGTCGAGCCCACTGCCTCAGAAGACTTCCTTTCATC22670.12497608805549447No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCCGGGCACGGTGGCTCA20960.11554913125907207No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA19370.10678371529046879No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCCGGGCGCGGTGGCTCAC19180.10573627564642185No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCTTTCCGGCGGTGACGAC18880.10408242357687408No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAATTCTGGACATTAATTA18690.10303498393282715No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAATTCTGGACATTAATT18330.1010503614493698No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA248050.0138.213618
GTGGTAT249550.0137.325136
GTATCAA250800.0137.186119
AAGCAGT251250.0136.825791
TGGTATC252150.0135.937687
CAGTGGT256500.0133.576174
AGTGGTA256700.0133.52825
AGCAGTG257150.0133.126542
GCAGTGG263400.0129.967683
AGAGTAC247600.028.14105815-19
ATCAACG248600.028.12022210-14
TCAACGC249300.028.08747110-14
CAACGCA250700.027.98805610-14
CAGAGTA249150.027.9659915-19
TACATGG246950.027.83137120-24
GTACATG248250.027.81903820-24
AGTACAT250800.027.66824320-24
GAGTACA248350.027.46574620-24
GCTAACG1050.00797772227.4265633
CGCAGAG255750.027.33436815-19
Produced by FastQC (version 0.11.5)