FastQCFastQC Report
Sat 18 Mar 2023
SRR4895526_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895526_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1115544
Sequences flagged as poor quality0
Sequence length150
%GC33

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AACTCACCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG17300.15508128769461357No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACTCAC1118850.0143.562181
ACTCACC1120250.0143.382782
CTCACCG396850.0143.031783
TCACCGA115900.0142.875174
TCACCTG137700.0142.372484
CTCACCT395550.0142.173163
CTCACCA332600.0142.045123
TCACCGG139950.0142.038424
TCACCGT141900.0141.557924
TCACCTA116000.0141.510684
TCACCAG112600.0141.179954
CACCTGA37050.0140.106865
CACCTAG40000.0140.033515
CACCGAT41850.0139.864495
ACCGGAT16300.0139.576346
CACCGTA45700.0139.267065
ACCGAAT13300.0139.121376
CACCGAA36200.0138.82235
TCACCAT122550.0138.705924
CACCTGG53150.0138.574955
Produced by FastQC (version 0.11.5)