FastQCFastQC Report
Sat 18 Mar 2023
SRR4895526_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895526_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1115544
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG63440.5686911497888026No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA52680.47223596738452267No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC51960.4657817172608163No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAATTCTGGACATTAATT20800.18645611468485332No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG19990.17919508329568354No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAATTCTGGACATTAATTA17720.15884626693344234No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTTTCTTGCTGCAGCAACGC17140.15364700988934546No Hit
GAGGAGGCTACTGGCGTCGAGCCCACTGCCTCAGAAGACTTCCTTTCATC14290.12809893648300738No Hit
CCACTGCCTCAGAAGACTTCCTTTCATCTGTTCCTACTGAGCTGGTCCCA11870.10640548467832735No Hit
GGTGGTCACTGCGTGGCCCTGTGTACCCGAGGAGGCTACTGGCGTCGAGC11470.10281979016515709No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA169850.0130.384868
AAGCAGT171150.0130.00091
GTGGTAT171200.0129.609026
GTATCAA171600.0129.390849
TGGTATC172400.0128.456317
CAGTGGT174650.0127.089994
AGTGGTA175450.0126.469465
AGCAGTG178400.0124.4352652
GCAGTGG180200.0123.066913
TCGTACG900.027.20202350-54
CAACGCA169900.027.0100310-14
ATCAACG169700.026.88913310-14
AGAGTAC169800.026.78873615-19
TCAACGC170700.026.77378710-14
CAGAGTA170250.026.7179315-19
GTACATG169450.026.69254720-24
TACGCAC2000.026.64198150-54
TACATGG170300.026.61005620-24
AGTACAT171300.026.48833820-24
CGCAGAG173950.026.16618215-19
Produced by FastQC (version 0.11.5)