FastQCFastQC Report
Sat 18 Mar 2023
SRR4895525_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895525_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2724217
Sequences flagged as poor quality0
Sequence length150
%GC33

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGCA2727850.0143.800162
CACGCAT982600.0143.685093
TTCACGC2730500.0143.66061
ACGCATA301500.0143.300324
ACGCATG328650.0143.26994
ACGCATT351650.0143.255774
CACGCAG936750.0142.993683
ACGCAAT297650.0142.976914
CACGCAA815000.0142.959263
ACGCAGT334850.0142.896274
CGCATAG103900.0142.329775
ACGCAGA273300.0142.148794
ACGCAAG275850.0142.061434
CGCAGTA107350.0141.779625
CGCAATG100850.0141.708365
CGCATAT107300.0141.644385
ACGCAGG330900.0141.53424
CGCATTA104800.0141.519675
CGCATGA94500.0141.478675
CGCATAA93200.0141.366355
Produced by FastQC (version 0.11.5)