FastQCFastQC Report
Sat 18 Mar 2023
SRR4895525_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895525_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2724217
Sequences flagged as poor quality0
Sequence length150
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA124390.456608265787931No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG69830.25633053460866007No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC61960.2274414997043187No Hit
GAGGAGGCTACTGGCGTCGAGCCCACTGCCTCAGAAGACTTCCTTTCATC52450.19253238637010195No Hit
GTCGAGCCCACTGCCTCAGAAGACTTCCTTTCATCTGTTCCTACTGAGCT40350.14811595405211847No Hit
GGTGGTCACTGCGTGGCCCTGTGTACCCGAGGAGGCTACTGGCGTCGAGC39840.1462438564916084No Hit
CCACTGCCTCAGAAGACTTCCTTTCATCTGTTCCTACTGAGCTGGTCCCA39690.14569323956204663No Hit
TGGAACTTTGCGTACTGGGCTGCTCACCAGCCCTGGTCCCGCGGTGGTCA32630.11977753607733892No Hit
CTCAGAAGACTTCCTTTCATCTGTTCCTACTGAGCTGGTCCCAGCCAGCA32150.11801556190274122No Hit
GAGCCCACTGCCTCAGAAGACTTCCTTTCATCTGTTCCTACTGAGCTGGT31600.1159966331610147No Hit
AGAAGACTTCCTTTCATCTGTTCCTACTGAGCTGGTCCCAGCCAGCAGTT31110.1141979511911129No Hit
GTGGCCCTGTGTACCCGAGGAGGCTACTGGCGTCGAGCCCACTGCCTCAG30140.11063729504661339No Hit
AAGACTTCCTTTCATCTGTTCCTACTGAGCTGGTCCCAGCCAGCAGTTCA29720.10909556764384042No Hit
GGCAGCCGCTGGAACTTTGCGTACTGGGCTGCTCACCAGCCCTGGTCCCG28980.10637919079133563No Hit
AGCCGCTGGAACTTTGCGTACTGGGCTGCTCACCAGCCCTGGTCCCGCGG28890.10604882063359856No Hit
CACTGCCTCAGAAGACTTCCTTTCATCTGTTCCTACTGAGCTGGTCCCAG28210.1035526905529185No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA367250.0136.719998
AAGCAGT369850.0136.197111
GTGGTAT368950.0136.148566
GTATCAA370500.0135.831599
TGGTATC372150.0134.939187
CAGTGGT377600.0133.105964
AGTGGTA377800.0133.073615
AGCAGTG377800.0132.949982
GCAGTGG390500.0128.607683
TCAACGC366600.028.17803810-14
ATCAACG366600.028.11519210-14
AGAGTAC367050.028.04180315-19
CAGAGTA368850.027.8854415-19
TACATGG366150.027.85229920-24
GTACATG370400.027.71155520-24
CAACGCA375600.027.56801810-14
AGTACAT377000.027.31808720-24
GCAGAGT376900.027.27456915-19
GAGTACA369600.027.0858220-24
CGCAGAG381150.026.98555415-19
Produced by FastQC (version 0.11.5)