FastQCFastQC Report
Sat 18 Mar 2023
SRR4895524_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895524_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2540025
Sequences flagged as poor quality0
Sequence length150
%GC35

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTGGTA2553900.0143.209272
GTGGTAA751750.0142.30743
GTGGTAG879300.0141.741323
GTGGTAT944400.0141.553473
TGGTGGT2612850.0139.978271
TGGTAAG254400.0139.578114
TGGTATA277000.0139.548454
TGGTAGA245950.0139.13374
TGGTAAT285500.0138.167714
TGGTAGG318050.0137.85844
TGGTATG324600.0137.804764
TGGTAGT326400.0136.62574
GGTATTA101850.0135.510335
GGTAGTA98500.0135.148765
GGTAATA83050.0134.457035
TGGTAAA229600.0134.4294
GGTATGA86950.0134.387945
GGTATAG98750.0133.931725
GGTATAA84950.0133.060045
GGTAGAA79800.0131.903355
Produced by FastQC (version 0.11.5)