FastQCFastQC Report
Sat 18 Mar 2023
SRR4895523_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895523_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3982325
Sequences flagged as poor quality0
Sequence length150
%GC34

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCTTCA3991150.0143.677082
GCTTCAG1385900.0143.378173
TGGCTTC4000250.0143.350231
GCTTCAA1189000.0143.257613
GCTTCAT1425650.0142.789373
CTTCAGA395000.0141.535684
CTTCAAG404700.0141.50574
CTTCAGT516750.0141.12624
CTTCATG498100.0141.047644
CTTCAAT445700.0139.537874
CTTCATA435900.0139.322024
CTTCAGG501050.0137.932464
CTTCATT529250.0136.024544
TTCAGAG138800.0134.866915
CTTCAAA374950.0134.664154
TTCATAG157250.0134.656075
TCATGAG52400.0134.653096
TCAGTGA51300.0134.452766
TTCATGG190550.0134.323565
TTCAGAT149400.0134.261675
Produced by FastQC (version 0.11.5)