FastQCFastQC Report
Sat 18 Mar 2023
SRR4895523_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895523_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3982325
Sequences flagged as poor quality0
Sequence length150
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG211400.5308456743234166No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA206910.5195708537098302No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC123550.3102458990665001No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTTTCTTGCTGCAGCAACGC59800.1501635351208151No Hit
GAGGAGGCTACTGGCGTCGAGCCCACTGCCTCAGAAGACTTCCTTTCATC50800.12756367197554191No Hit
GGTGGTCACTGCGTGGCCCTGTGTACCCGAGGAGGCTACTGGCGTCGAGC40310.10122227593177353No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA645750.0137.77728
GTGGTAT649250.0137.123186
AAGCAGT652900.0136.83251
TGGTATC651200.0136.613077
GTATCAA655700.0136.07089
AGTGGTA664500.0134.008765
AGCAGTG666200.0133.69012
CAGTGGT667400.0133.512774
GCAGTGG688250.0129.39653
TCAACGC647400.028.1480410-14
ATCAACG646850.028.14303410-14
AGAGTAC650150.027.98253615-19
CAGAGTA652300.027.88589115-19
TACATGG648000.027.86010620-24
CAACGCA657050.027.82229810-14
GTACATG651350.027.77208920-24
AGTACAT660300.027.48506520-24
CGCAGAG664150.027.377515-19
GAGTACA652100.027.23445520-24
GCAGAGT668250.027.1578115-19
Produced by FastQC (version 0.11.5)