FastQCFastQC Report
Sat 18 Mar 2023
SRR4895522_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895522_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2241452
Sequences flagged as poor quality0
Sequence length150
%GC31

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGAACA2247400.0143.614351
GGAACAA2250100.0143.442032
GAACAAT834300.0143.287953
GAACAAG796950.0143.236713
AACAATG287700.0141.167724
AACAAGT291200.0140.633064
GAACAAA630700.0140.513853
AACAAGG287100.0140.183784
ACAATGG102650.0139.92755
ACAAGTG100300.0139.616855
ACAATTG110700.0139.31285
ACAATAG92600.0139.01955
ACAAGTA89550.0138.367615
ACAATGA82350.0137.70075
AACAAGA238400.0136.656894
AACAAAG237600.0136.450364
ACAATGT108600.0136.17265
ACAAGGT108300.0135.885035
ACAAGGA83300.0135.525255
AACAATA260550.0135.429354
Produced by FastQC (version 0.11.5)