FastQCFastQC Report
Sat 18 Mar 2023
SRR4895522_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895522_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2241452
Sequences flagged as poor quality0
Sequence length150
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA137850.6150031318984301No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG74590.33277536168519334No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC52760.23538313557461857No Hit
GAGGAGGCTACTGGCGTCGAGCCCACTGCCTCAGAAGACTTCCTTTCATC47000.20968550743000522No Hit
GTCGAGCCCACTGCCTCAGAAGACTTCCTTTCATCTGTTCCTACTGAGCT36880.16453620242592748No Hit
GGTGGTCACTGCGTGGCCCTGTGTACCCGAGGAGGCTACTGGCGTCGAGC35760.1595394413978082No Hit
CCACTGCCTCAGAAGACTTCCTTTCATCTGTTCCTACTGAGCTGGTCCCA35170.15690721907049537No Hit
CTCAGAAGACTTCCTTTCATCTGTTCCTACTGAGCTGGTCCCAGCCAGCA31960.14258614505240352No Hit
AGAAGACTTCCTTTCATCTGTTCCTACTGAGCTGGTCCCAGCCAGCAGTT28850.12871121041182235No Hit
AAGACTTCCTTTCATCTGTTCCTACTGAGCTGGTCCCAGCCAGCAGTTCA28230.1259451462712563No Hit
GAGCCCACTGCCTCAGAAGACTTCCTTTCATCTGTTCCTACTGAGCTGGT28100.12536516508049247No Hit
AGCCGCTGGAACTTTGCGTACTGGGCTGCTCACCAGCCCTGGTCCCGCGG27840.12420520269896478No Hit
TGGAACTTTGCGTACTGGGCTGCTCACCAGCCCTGGTCCCGCGGTGGTCA27130.12103761311863916No Hit
GTGGCCCTGTGTACCCGAGGAGGCTACTGGCGTCGAGCCCACTGCCTCAG26950.12023456223912No Hit
CACTGCCTCAGAAGACTTCCTTTCATCTGTTCCTACTGAGCTGGTCCCAG25990.11595162421501777No Hit
CGCTGGAACTTTGCGTACTGGGCTGCTCACCAGCCCTGGTCCCGCGGTGG25470.11363169945196239No Hit
GGCAGCCGCTGGAACTTTGCGTACTGGGCTGCTCACCAGCCCTGGTCCCG25400.11331940188770494No Hit
GAAGACTTCCTTTCATCTGTTCCTACTGAGCTGGTCCCAGCCAGCAGTTC24230.10809957117083034No Hit
GCTGGAACTTTGCGTACTGGGCTGCTCACCAGCCCTGGTCCCGCGGTGGT24110.10756420391781756No Hit
AGGATCACAGGCTCGGGTCGCTGCAGACGCTTTCAGTGGGTTGACGGCAG23810.1062257857852856No Hit
GTACTGGGCTGCTCACCAGCCCTGGTCCCGCGGTGGTCACTGCGTGGCCC23670.10560119065677069No Hit
AGACTTCCTTTCATCTGTTCCTACTGAGCTGGTCCCAGCCAGCAGTTCAG23570.10515505127926006No Hit
AGACGCTTTCAGTGGGTTGACGGCAGCCGCTGGAACTTTGCGTACTGGGC23540.10502120946600686No Hit
AGCCCTGGTCCCGCGGTGGTCACTGCGTGGCCCTGTGTACCCGAGGAGGC23500.10484275371500261No Hit
CCTGGTCCCGCGGTGGTCACTGCGTGGCCCTGTGTACCCGAGGAGGCTAC23320.10403970283548343No Hit
CAGAAGACTTCCTTTCATCTGTTCCTACTGAGCTGGTCCCAGCCAGCAGT23210.10354894952022173No Hit
CGAGGAGGCTACTGGCGTCGAGCCCACTGCCTCAGAAGACTTCCTTTCAT23150.10328126589371532No Hit
AGCCCACTGCCTCAGAAGACTTCCTTTCATCTGTTCCTACTGAGCTGGTC22940.10234437320094296No Hit
TGCTCACCAGCCCTGGTCCCGCGGTGGTCACTGCGTGGCCCTGTGTACCC22840.1018982338234323No Hit
ACCAGCCCTGGTCCCGCGGTGGTCACTGCGTGGCCCTGTGTACCCGAGGA22740.10145209444592167No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA297900.0137.662378
GTGGTAT299200.0137.232676
AAGCAGT300850.0136.98871
TGGTATC300300.0136.658077
GTATCAA301950.0136.435879
AGCAGTG304200.0134.912062
AGTGGTA308000.0133.311755
CAGTGGT308350.0133.183784
GCAGTGG315050.0130.194373
ATCAACG298400.028.09918210-14
TCAACGC299950.028.04999410-14
AGAGTAC299400.028.02950715-19
CAACGCA302050.027.9693910-14
CAGAGTA300950.027.90906715-19
TACATGG299600.027.78564520-24
GTACATG300150.027.74912320-24
CGCAGAG305250.027.44987315-19
AGTACAT304650.027.44322820-24
GAGTACA300750.027.24847820-24
GCAGAGT307300.027.24801415-19
Produced by FastQC (version 0.11.5)