FastQCFastQC Report
Sat 18 Mar 2023
SRR4895521_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895521_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1595516
Sequences flagged as poor quality0
Sequence length150
%GC31

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGAAGAG1600150.0143.575941
GAAGAGA1606100.0143.044052
AAGAGAT583450.0142.08173
AAGAGAG564250.0141.455183
AAGAGAA474850.0140.264533
AGAGATG225050.0127.710644
AGAGATA192650.0127.139614
GAGATAG66950.0126.5731055
AGAGAGT224250.0125.7583164
AGAGAAT186300.0125.7149054
AGAGAGA182450.0125.3291854
AGAGAAG185650.0125.146744
GAGATTA69350.0124.892045
GAGATTG82800.0124.6038365
AGAGAGG227500.0124.404834
AGAGATT237000.0123.792644
GAGAATG66350.0122.943225
GAGAATA54950.0122.50665
GAGAGTA71050.0121.903775
GAGAAGT68100.0121.898335
Produced by FastQC (version 0.11.5)