FastQCFastQC Report
Sat 18 Mar 2023
SRR4895521_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895521_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1595516
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA100240.6282607006134693No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG50260.3150078093858037No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC33890.212407772783225No Hit
GAGGAGGCTACTGGCGTCGAGCCCACTGCCTCAGAAGACTTCCTTTCATC28080.1759932210018577No Hit
GTCGAGCCCACTGCCTCAGAAGACTTCCTTTCATCTGTTCCTACTGAGCT22650.14196034386367795No Hit
CCACTGCCTCAGAAGACTTCCTTTCATCTGTTCCTACTGAGCTGGTCCCA22230.13932796662647068No Hit
GGTGGTCACTGCGTGGCCCTGTGTACCCGAGGAGGCTACTGGCGTCGAGC20840.1306160514842847No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAATTCTGGACATTAATTA19190.12027456948097041No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAATTCTGGACATTAATT18840.11808092178329768No Hit
CTCAGAAGACTTCCTTTCATCTGTTCCTACTGAGCTGGTCCCAGCCAGCA17890.11212673517532887No Hit
AGAAGACTTCCTTTCATCTGTTCCTACTGAGCTGGTCCCAGCCAGCAGTT17070.10698733199792418No Hit
TGGAACTTTGCGTACTGGGCTGCTCACCAGCCCTGGTCCCGCGGTGGTCA16810.10535776513679587No Hit
GAGCCCACTGCCTCAGAAGACTTCCTTTCATCTGTTCCTACTGAGCTGGT16690.10460565735473666No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCTTTCCGGCGGTGACGAC16600.10404157651819224No Hit
AGCCGCTGGAACTTTGCGTACTGGGCTGCTCACCAGCCCTGGTCCCGCGG16230.10172257752350965No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTTTCTTGCTGCAGCAACGC15960.10003033501387638No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA209000.0137.899228
GTGGTAT209300.0137.529566
AAGCAGT211050.0136.969121
GTATCAA211850.0136.349939
TGGTATC212350.0135.655937
AGTGGTA214000.0134.54275
CAGTGGT215950.0133.294454
AGCAGTG218900.0131.695452
GCAGTGG223200.0129.09383
TCAACGC209550.028.14652410-14
AGAGTAC209850.028.1339115-19
CAACGCA210850.028.10274310-14
ATCAACG210000.028.09992210-14
TACATGG209050.028.00118820-24
CAGAGTA211400.027.90719215-19
GTACATG211250.027.70276520-24
CGCAGAG213800.027.53330815-19
GAGTACA210350.027.40369820-24
AACGCAG216350.027.38166610-14
AGTACAT215100.027.33411820-24
Produced by FastQC (version 0.11.5)