FastQCFastQC Report
Sat 18 Mar 2023
SRR4895520_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895520_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1694917
Sequences flagged as poor quality0
Sequence length150
%GC30

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCACA1702750.0143.328492
TCTTCAC1708050.0142.883761
TTCACAG587050.0142.569693
TCACATG204500.0142.161624
TTCACAT618200.0141.884383
TCACAGG206200.0141.722814
TCACAAG177400.0141.557284
TCACAGT220600.0141.316134
TCACAGA163550.0140.778814
TTCACAA515450.0140.151233
TCACATA183900.0139.842474
TCACAAT188200.0139.784274
CACATGG75850.0139.626045
CACATAG66150.0139.421145
TCACATT234250.0139.382114
CACAGTG76100.0138.315895
CACATTG84150.0138.260015
CACAGAT60200.0138.251775
CACATGA59700.0137.96255
CACAGTA66400.0137.811945
Produced by FastQC (version 0.11.5)