FastQCFastQC Report
Sat 18 Mar 2023
SRR4895520_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895520_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1694917
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG122640.7235752547174876No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA107630.6350163459331637No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC104720.6178473636172155No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCACGGTGGCTCATGCCT36640.21617577733894933No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAATTCTGGACATTAATT24460.1443138513567331No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAATTCTGGACATTAATTA21910.12926886685306713No Hit
GAGGAGGCTACTGGCGTCGAGCCCACTGCCTCAGAAGACTTCCTTTCATC21500.12684986934463457No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA276700.0137.306358
AAGCAGT277500.0137.070221
GTGGTAT277750.0136.583926
GTATCAA279950.0136.072379
TGGTATC280500.0135.347557
AGCAGTG285250.0133.0182
CAGTGGT286150.0132.649964
AGTGGTA289300.0131.205615
GCAGTGG289650.0131.047073
CAACGCA277650.028.14004310-14
ATCAACG277300.028.06132310-14
AGAGTAC276750.028.03954115-19
TCAACGC278500.027.99211310-14
CAGAGTA277850.027.91298315-19
GTACATG278000.027.78075820-24
TACATGG277100.027.74626220-24
CGCAGAG281300.027.59624115-19
AGTACAT281500.027.45069720-24
GAGTACA277250.027.28455720-24
GCAGAGT285300.027.11848315-19
Produced by FastQC (version 0.11.5)