FastQCFastQC Report
Sat 18 Mar 2023
SRR4895519_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895519_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1725752
Sequences flagged as poor quality0
Sequence length150
%GC32

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTCTA1727500.0143.84922
CGTCTAG590200.0143.67793
CGTCTAA520650.0143.635543
CGTCTAT617650.0143.528813
TCCGTCT1732950.0143.39681
GTCTAAG179100.0143.191094
GTCTAGA167700.0143.007634
GTCTATA181150.0142.7634
GTCTAGG208000.0142.61054
GTCTATG215900.0142.32774
GTCTAAT189950.0142.327334
GTCTAGT216600.0142.299854
GTCTAAA155200.0141.443624
GTCTATT225800.0140.934074
TCTAAGG68750.0140.853365
TCTAGAG56300.0140.670145
TCTAGTA69400.0140.156595
TCTAGGA62150.0140.056345
CTAGATG22150.0139.777596
TCTAGAT61500.0139.663515
Produced by FastQC (version 0.11.5)