FastQCFastQC Report
Sat 18 Mar 2023
SRR4895519_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895519_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1725752
Sequences flagged as poor quality0
Sequence length150
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA85110.49317630806743956No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG80580.46692688173039926No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC63610.36859293803512905No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCCGGGCGCGGTGGCTCAC48250.27958826065390624No Hit
GAGGAGGCTACTGGCGTCGAGCCCACTGCCTCAGAAGACTTCCTTTCATC28690.16624636680125535No Hit
GTCGAGCCCACTGCCTCAGAAGACTTCCTTTCATCTGTTCCTACTGAGCT23080.1337387990858478No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAATTCTGGACATTAATT22890.13263782976928318No Hit
GGTGGTCACTGCGTGGCCCTGTGTACCCGAGGAGGCTACTGGCGTCGAGC22470.1302041081221404No Hit
CCACTGCCTCAGAAGACTTCCTTTCATCTGTTCCTACTGAGCTGGTCCCA22270.12904519305207238No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCGGGCGCGGTGGCTCACG21950.12719092893996356No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAATTCTGGACATTAATTA21800.12632174263741255No Hit
CTCAGAAGACTTCCTTTCATCTGTTCCTACTGAGCTGGTCCCAGCCAGCA19190.11119790097302511No Hit
AGAAGACTTCCTTTCATCTGTTCCTACTGAGCTGGTCCCAGCCAGCAGTT18010.10436030205962386No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCATGGTGGCTCACACCT17830.10331727849656265No Hit
TGGAACTTTGCGTACTGGGCTGCTCACCAGCCCTGGTCCCGCGGTGGTCA17750.10285371246853546No Hit
AAGACTTCCTTTCATCTGTTCCTACTGAGCTGGTCCCAGCCAGCAGTTCA17280.10013026205387564No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA238450.0137.531518
GTGGTAT241350.0136.177286
GTATCAA242000.0135.999
AAGCAGT242800.0135.909591
TGGTATC243400.0134.704997
AGTGGTA249000.0132.022435
AGCAGTG249200.0131.927952
CAGTGGT249500.0131.844424
GCAGTGG254550.0129.066543
CAACGCA239400.028.22718610-14
AGAGTAC238700.028.20203415-19
TCAACGC240500.028.03820610-14
ATCAACG239900.028.01229710-14
CAGAGTA241100.027.92727315-19
GTACATG240150.027.87763220-24
TACATGG240400.027.8366620-24
CGCAGAG243450.027.61629115-19
AGTACAT243350.027.55838820-24
GAGTACA239650.027.3649420-24
GCAGAGT246250.027.30227915-19
Produced by FastQC (version 0.11.5)