FastQCFastQC Report
Sat 18 Mar 2023
SRR4895518_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895518_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2042889
Sequences flagged as poor quality0
Sequence length150
%GC29

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCAGC2044700.0143.862811
ATCAGCA2045500.0143.806552
TCAGCAG714300.0143.589923
TCAGCAT723300.0143.49543
TCAGCAA610150.0143.013723
CAGCATG251500.0142.6194
CAGCAAT217650.0142.504554
CAGCATT265750.0142.448874
CAGCATA209200.0142.306754
CAGCAGT260900.0142.226994
CAGCAAG210600.0141.907734
CAGCAGG254550.0141.617254
AGCATTG91250.0141.310495
CAGCAGA205050.0141.219274
AGCAGTA78600.0141.153555
AGCATAG73450.0140.75845
AGCATGG91300.0140.444535
AGCATGA70800.0140.433945
AGCATGT93050.0140.356525
AGCAGGT94150.0140.322545
Produced by FastQC (version 0.11.5)