FastQCFastQC Report
Sat 18 Mar 2023
SRR4895518_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895518_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2042889
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA135940.6654301824523996No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG81110.39703576650517963No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC64270.31460348555403644No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTTTCTTGCTGCAGCAACGC29720.14548024880451166No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCCGGGCGCGGTGGCTCA26510.12976720712676998No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCCGGGCGCGGTGGCTCAC24750.1211519568610923No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAATTCTGGACATTAATT21150.10352985404493342No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAATTCTGGACATTAATTA20470.10020123462410341No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA299500.0135.89368
GTGGTAT299900.0135.616326
AAGCAGT302000.0135.177251
TGGTATC302050.0134.698677
GTATCAA303600.0134.437859
CAGTGGT307250.0132.418994
AGTGGTA307600.0132.22155
AGCAGTG311600.0130.59672
GCAGTGG317750.0128.069023
ATCAACG298600.027.9068310-14
TCAACGC300100.027.80572910-14
AGAGTAC298500.027.76195115-19
CAGAGTA300450.027.60573215-19
CAACGCA303250.027.58812310-14
GTACATG299900.027.49391620-24
TACATGG300450.027.44358620-24
AGTACAT304900.027.13750520-24
GAGTACA299100.027.07156820-24
CGCAGAG307750.027.0351315-19
CGACCTA4550.026.90118245-49
Produced by FastQC (version 0.11.5)