FastQCFastQC Report
Sat 18 Mar 2023
SRR4895517_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895517_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1886377
Sequences flagged as poor quality0
Sequence length150
%GC33

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCACAT1890150.0143.705721
CCACATA1892900.0143.496932
CACATAG673700.0143.482223
ACATAGG244700.0143.08314
CACATAT666850.0142.667223
ACATAGT229750.0142.585024
ACATATG240300.0142.407234
ACATAAG198300.0142.288764
CACATAA558800.0142.114093
CATATGG89350.0141.900155
CATAGGT87200.0141.84855
ACATAGA201600.0141.5314
CATAGGA69650.0141.514315
CATAGTG78100.0141.137445
CATAAGG73500.0140.762655
CATAGAG72350.0140.711275
CATATAG76200.0140.688235
CATAGGG90800.0140.189165
CATATGA68500.0140.106285
CATAAGT69900.0140.081165
Produced by FastQC (version 0.11.5)