FastQCFastQC Report
Sat 18 Mar 2023
SRR4895517_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895517_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1886377
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG130020.6892577676678627No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA101770.5394997924593016No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC80550.4270090231168001No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCCGGGCGCGGTGGCTCAC28690.15209048880472992No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT28320.15012905691704256No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCGGGCGCGGTGGCTCACG25750.1365050570485115No Hit
GAGGAGGCTACTGGCGTCGAGCCCACTGCCTCAGAAGACTTCCTTTCATC24740.13115087811185144No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAATTCTGGACATTAATTA20480.10856790556712684No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCCAGGCGTGGTGGCTCA20310.10766670713224344No Hit
CCACTGCCTCAGAAGACTTCCTTTCATCTGTTCCTACTGAGCTGGTCCCA19810.10501612350023351No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAATTCTGGACATTAATT19780.10485708848231293No Hit
GTCGAGCCCACTGCCTCAGAAGACTTCCTTTCATCTGTTCCTACTGAGCT19730.10459203011911192No Hit
GGTGGTCACTGCGTGGCCCTGTGTACCCGAGGAGGCTACTGGCGTCGAGC18900.10019206128997545No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT298700.0137.731581
GGTATCA298550.0137.1468
GTGGTAT299700.0136.763896
GTATCAA301300.0136.372189
TGGTATC301400.0135.849177
CAGTGGT305700.0134.150274
AGCAGTG307750.0133.377172
AGTGGTA308900.0132.783875
GCAGTGG313950.0130.57923
ATCAACG297900.028.20926510-14
CAACGCA299100.028.20200310-14
TCAACGC299550.028.10675810-14
AGAGTAC299500.028.08755915-19
CAGAGTA300800.027.96617115-19
TACATGG299450.027.85270120-24
GTACATG300950.027.7569420-24
CGCAGAG304350.027.5831915-19
AGTACAT304450.027.54190320-24
GAGTACA299300.027.34704820-24
GCAGAGT307550.027.29619415-19
Produced by FastQC (version 0.11.5)