FastQCFastQC Report
Sat 18 Mar 2023
SRR4895516_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895516_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1533134
Sequences flagged as poor quality0
Sequence length150
%GC30

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGATG1536350.0143.68971
AGGATGA1537000.0143.628922
GGATGAA470500.0142.969043
GGATGAT596200.0142.859153
GGATGAG477150.0142.606113
GATGATA191300.0137.596224
GATGAAT184600.0136.973984
GATGAAG154800.0136.692264
GATGAGA147250.0136.562334
GATGATG195200.0136.359344
GATGAGT188250.0134.700644
GATGAGG166150.0134.374454
ATGATAG64200.0133.340525
GATGAAA155450.0132.647054
ATGATGA59350.0132.46995
GATGATT242750.0132.101044
ATGAGTA56050.0132.04835
ATGATTA66250.0131.931385
ATGAAGG52900.0131.336945
ATGAATG62850.0131.04975
Produced by FastQC (version 0.11.5)