FastQCFastQC Report
Sat 18 Mar 2023
SRR4895516_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895516_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1533134
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA122690.800256207219982No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG44590.29084215730653684No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC35660.23259545480042843No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTTTCTTGCTGCAGCAACGC24130.157390025920761No Hit
GAGGAGGCTACTGGCGTCGAGCCCACTGCCTCAGAAGACTTCCTTTCATC22230.1449971104939294No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAATTCTGGACATTAATT21030.13717000601382526No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCTGGGCGCAGTGGCTCA21020.13710478014315775No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCCGGGCGTGGTGGCTCAC20670.13482187466979403No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCGCGGTGGCTCACGCCT20600.13436529357512128No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGTTTCTTGCTGCAGCAACG19520.12732089954302755No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAATTCTGGACATTAATTA19220.12536412342300152No Hit
GTCGAGCCCACTGCCTCAGAAGACTTCCTTTCATCTGTTCCTACTGAGCT17280.1127103045134998No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA17060.11127533535881402No Hit
GGTGGTCACTGCGTGGCCCTGTGTACCCGAGGAGGCTACTGGCGTCGAGC17040.11114488361747897No Hit
CCACTGCCTCAGAAGACTTCCTTTCATCTGTTCCTACTGAGCTGGTCCCA16530.10781836421343471No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCCAGGCGCTGTGGCTCAC16080.10488320003339563No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA211050.0136.693738
AAGCAGT211900.0136.081881
GTGGTAT211900.0136.009496
TGGTATC215300.0133.928537
GTATCAA216150.0133.635019
AGTGGTA218400.0132.060495
CAGTGGT218850.0131.756064
AGCAGTG218950.0131.535752
GCAGTGG229500.0125.489113
ATCAACG211400.028.1176710-14
AGAGTAC211300.028.10371615-19
TCAACGC213250.027.94126110-14
TACATGG210900.027.86343220-24
CAGAGTA213050.027.85935415-19
CAACGCA215200.027.7549910-14
GTACATG213150.027.63686420-24
AGTACAT214100.027.55458520-24
GCAGAGT217850.027.21246715-19
CGCAGAG218800.027.15354215-19
GAGTACA212150.027.0273120-24
Produced by FastQC (version 0.11.5)