FastQCFastQC Report
Sat 18 Mar 2023
SRR4895514_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895514_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1332722
Sequences flagged as poor quality0
Sequence length150
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCA1335450.0143.596422
GTCTGTC1335800.0143.55881
CTGTCAT450850.0143.429963
CTGTCAG398450.0143.387153
CTGTCAA366150.0142.988863
CTGTCAC124050.0141.97883
TGTCAGA111100.0141.949984
TGTCATG144450.0141.850654
TGTCATA136650.0141.786734
TGTCAAT122800.0141.724984
TGTCAGT126300.0141.614154
TGTCAGG138100.0140.92324
TGTCAAG121850.0140.82244
GTCATGA40300.0140.322195
GTCATAG46300.0140.163335
GTCAGAT41350.0139.716895
GTCATGG49800.0139.414155
GTCAATA36300.0139.135965
TGTCATT139700.0139.000184
GTCATAT46400.0138.930925
Produced by FastQC (version 0.11.5)