FastQCFastQC Report
Sat 18 Mar 2023
SRR4895513_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895513_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1287942
Sequences flagged as poor quality0
Sequence length150
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTAGA1288850.0143.817442
GTCGTAG1289100.0143.789541
CGTAGAT445000.0143.583923
CGTAGAG374500.0143.40473
CGTAGAA354150.0143.334243
CGTAGAC119000.0142.835023
GTAGATA135000.0141.098364
GTAGAAG118450.0140.642874
GTAGATG143650.0140.416844
GTAGAAT123600.0139.731574
GTAGAGT121850.0139.671374
GTAGAGG124850.0139.312424
GTAGACG31900.0138.960634
GTAGATT132650.0138.552844
GTAGACC35600.0138.26364
GTAGAGA104700.0138.081344
GTAGACT28200.0137.288764
GTAGAGC35550.0136.028994
GTAGACA27950.0134.912224
TAGATGG51050.0134.619935
Produced by FastQC (version 0.11.5)