FastQCFastQC Report
Sat 18 Mar 2023
SRR4895513_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895513_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1287942
Sequences flagged as poor quality0
Sequence length150
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA107100.8315591851185845No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG26550.2061428232016659No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC23200.18013233515173818No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTTTCTTGCTGCAGCAACGC17460.13556511085126505No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA167700.0135.410758
GTGGTAT168750.0134.312276
TGGTATC169150.0134.249977
AAGCAGT170100.0133.763691
GTATCAA170750.0133.034139
CAGTGGT173300.0130.952034
AGTGGTA174150.0130.188875
AGCAGTG174850.0130.047492
GCAGTGG180600.0125.7873843
AATACGC800.002110674535.9879264
ATCAACG167800.027.6150510-14
TCAACGC168550.027.58611710-14
CAACGCA169250.027.48052810-14
AGAGTAC168550.027.47508815-19
CAGAGTA168900.027.40963215-19
GTACATG169450.027.33319320-24
TACATGG170250.027.15401620-24
AGTACAT171050.027.00176620-24
CGCAGAG172300.026.88546615-19
GAGTACA168900.026.83403220-24
Produced by FastQC (version 0.11.5)