FastQCFastQC Report
Sat 18 Mar 2023
SRR4895512_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895512_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences608123
Sequences flagged as poor quality0
Sequence length150
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACGCA608550.0143.846941
TACGCAA608600.0143.835112
ACGCAAT209200.0143.678133
ACGCAAG188300.0143.0363
CGCAAGT55650.0142.789344
CGCAATA60550.0142.764664
CGCAATG72400.0142.462144
CGCAATT61600.0141.616474
CGCAAGA52550.0141.350984
CGCAAGG68250.0141.211434
GCAATAG21550.0140.94745
CGCAAAT51100.0140.854574
ACGCAAA158250.0140.633133
GCAAGAT18900.0140.525915
CGCAACT13700.0140.275744
GCAAGTG19300.0140.224015
ACGCAAC59250.0139.944553
GCAATAT19550.0139.903535
GCAATTG23700.0139.09425
GCAAGTA17150.0138.91715
Produced by FastQC (version 0.11.5)